Diversity of Streptococcus spp. and genomic characteristics of Streptococcus uberis isolated from clinical mastitis of cattle in Bangladesh

Introduction Streptococci are the major etiology in mastitis dairy cattle, a cause of huge economic losses industries. This study was aimed to determine diversity Streptococcus spp. isolated from clinical cattle reared Bangladesh. Methods A total 843 lactating four prominent farms and one community were purposively included this where 80 positive (CM) based on gross changes udder (redness, swelling, sensitive udder) and/or milk (flakes clots). Milk samples collected all eighty with (CCM) twenty five apparently healthy (AHC). Samples enriched Luria Bertani broth (LB) hundred microliter enrichment culture spread onto selective media for isolation Staphylococcus spp., Enterococcus Escherichia coli Corynebacterium pathogen associated mastitis. Isolates recovered further confirmed by species specific PCR. Results Discussion Out 105 examined 56.2% (59/105), 17.14% (18/105), 9.52% (10/105) 22.9% (24/105) , faecalis E. respectively. then directed determination through sequencing 16S rRNA . eighteen CCM (22.5%) but none AHC cultural molecular examination. Sequencing phylogenetic analysis identified 55.6, 33.3, 5.6 5.6% isolates as uberis agalactiae hyovaginalis urinalis Considering high prevalence worldwide increasing trend S. mastitis, in-depth characterization performed whole genome sequencing. Five strain subjected WGS two novel ST types identified, indicating presence at least different genotypes areas. On virulence profiling, harbored 35 putative genes probably intramammary infection (IMI) potential pathogen. Overall findings suggest that encountered bovine is diverse might be predominantly CM The carries an array factors need investigated genotypically phenotypically identify trait governing fitness bacterium. Moreover, genomic information could used development new tools gene profiling